Our affinity purified Goat anti-Mouse IgG1 isotype-specific secondary antibody is highly cross-adsorbed against against other mouse IgG subclasses (IgG2a, IgG2b, IgG3), and human, bovine and rabbit serum proteins and conjugated to biotin for convenient use in applications such as immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), flow cytometry (FC) and ELISA.